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PCR conditions and primers for plasmid and endogenous gene analysis.
| PCR | Primer | Sequence 5’→3’ | Amplicon Size | T Annealing (°C) |
|---|---|---|---|---|
| pVax1-vhsG | F1_(pVAX1)_VHSV R1_pVAX1_(VHSV) |
CAATGGGAGTTTGTTTTGGCACCAA TGCAGTTGGAGGGATGAGTGTATAG |
370 | 62 |
| pVax1-ihnG | F1_(pVAX1)_IHNV R1_pVAX1_(IHNV) |
GGCTAACTAGAGAACCCACTGCTTA GGATAGGCAATTAGTCCCCTGTTCT |
327 | 58 |
| Endogenous gene | B-Glob_FOR B-Glob_REV |
TCCATGGACTCAGAGACACTTC GACACAACGACAGCCAGGAA |
443 | 57 |
