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. 2022 Dec 17;23(24):16135. doi: 10.3390/ijms232416135

Figure 6.

Figure 6

Phenotype of LlERF110-silenced plants under heat stress and expression of HSFs and HSPs in silenced plants. (A) The phenotypes of the LlERF110-silenced lines recovered at room temperature at 1 d, 7 d, and 16 d after treatment with heat stress at 42 °C for 24 h. Bar: 10 cm. (B) Chlorophyll content in leaves of LlERF110-silenced plants (BSMV: LlERF110-1 and BSMV: LlERF110-13) recovered at room temperature for 8 d after heat stress. (C) Expression of LlERF110 in silenced lines (BSMV: LlERF110-1 and BSMV: LlERF110-13) using qRT-PCR. (D) Expression of Hsfs in LlERF110 silenced lines. (E) Expression of Hsps in LlERF110-silenced lines (BSMV: LlERF110-1 and BSMV: LlERF110-13). 18S rRNA was used as a control. Three independent experiments were performed, each with three technical replicates, the results of one experiment are shown. T-test analysis of variance was employed to identify treatment means that differed statistically. Samples with different stars are significantly different; * p < 0.05, ** p < 0.01, and *** p < 0.001; ns means non-significance.