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Kinetic parameters of immobilized and free porcine lipase (PPL) in various reaction media using pNPP at different concentrations.
| Medium | KM (mM) | VMAX (mM min−1) |
Kcat (min−1) |
Kcat/KM (min mM−1) |
|---|---|---|---|---|
| Enzyme only | 0.78 ± 0.03 | 0.43 ± 0.03 | 4.01 ± 0.03 | 5.14 ± 0.01 |
| Enzyme/AC | 0.17 ± 0.02 | 0.42 ± 0.03 | 3.71 ± 0.02 | 21.82 ± 0.03 |
| Enzyme/DES/AC | 4.79 ± 0.03 | 2.80 ± 0.02 | 49.07 ± 0.01 | 10.24 ± 0.03 |
