Figure 7.

Dynamic transcriptomic profiles of cells undergoing differentiation using EBS and 2DF protocols (inter-protocol differences). The cells were differentiated, and RNA was isolated as described in Figure 6. (a) Dynamic changes in the expression of selected genes associated with (i) cell pluripotency (DNMT3B, LIN28A, POU5F1 and SOX2); (ii) mesoderm (BMP4 and HAND1); (iii) hemogenic endothelium and hematopoietic precursors (CD34, DLL4 and RUNX1); and (iv) myeloid cells/macrophages (CD14, CD33, CD68 and CD84). (b) Inter-protocol DEGs identified by applying DESeq2 to all RNA-seq data obtained in three differentiation experiments in both protocols at all differentiation time points. For each cluster, selected GO terms are listed.