Detection of toxin-encoding genes in strain EC7372 by PCR analysis. Lanes 1, 100-bp DNA ladder; 2 to 5, PCR with hlyA primers on MG1655, SE124 (hly+), EC7372, and IH111-28; 6 to 9, PCR with cnf primers on MG1655, J96 (cnf+), EC7372, and IH111-28; 10 to 13, PCR with cdt primers on MG1655, DH5α/pOMEO (cdt+), EC7372, and IH111-28.