FIG. 7.
Detection by PCR of PAICFT073 sequences in strain EC7372. (A) Schematic representation of PAICFT073 (26) and positions of the primers complementary to PAICFT073 sequences used to amplify DNA fragments in EC7372. ORFs, open reading frames. (B) Amplification in EC7372 of DNA fragments using primers complementary to PAICFT073 sequences. Lane 1; 1-kb DNA ladder. PCR fragments of the expected sizes were amplified from EC7372 with PAICFT073 right-junction primers (lane 2), PAICFT073 left-junction primers (lane 3), papA primers (lane 4), L6 primers (lane 5), hlyD primers (lane 6), hp1-hp4 primers (lane 7), R9-R15 primers (lane 8), and R4 primers (lane 9). Lane 10, 100-bp DNA ladder.