Figure 1.

High-performance liquid chromatography (HPLC) analysis and experimental design for middle cerebral artery occlusion (MCAO) mice model: (A) HPLC chromatogram of the glycyrrhizic acid standards and its chemical structure (upper panel) and the methanolic extract of GR (GRex) (lower panel); conditions used: HPLC, YL-9100 system; column, ZORBAX Eclipse XBD-C18; mobile phase, a mixture of acetonitrile and 1% acetic acid in water (40:60, v/v); wavelength, 254 nm; column temperature, 25 °C; flow rate, 1.0 mL/min; injection volume, 10 μL; (B,C) ischemic stroke model in mice; mice were adapted for seven days at animal facilities. The MCAO was maintained for 90 min, and it was confirmed that relative cerebral blood flow (rCBF) was continuously maintained under 20%. After MCAO, GRex was administered, and biological indicators of experimental animals were measured at 22.5 h after reperfusion; CCAL, common carotid artery ligation.