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Evidence for diversifying selection near the 3′ end of the bfpA gene. A sliding-window plot of the proportions of synonymous and nonsynonymous sites (pS and pN, respectively) that have mutated among eight bfpA alleles is shown. The difference (pN − pS) is a measure of the level of selective constraint on various parts of the molecule. Note that pN − pS > 0 for codons 143 to 174 (indicated by the arrow), a pattern consistent with the effect of diversifying selection.
