TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequencea | Annealing sitea |
|---|---|---|
| Coding strand | ||
| Donne-6 | 5′-TCTTGGTGCTTGCGTGTC-3′ | 19–208 |
| Donne-28 | 5′-CGCGGATCCATGGTTTCTAAAATCATGAAT-3′ | 274–294 |
| Donne-362 | 5′-AGGTCTGTCTTTGATTGAA-3′ | 309–327 |
| Donne-423 | 5′-GATTATTCCGTGACCTATT-3′ | 223–241 |
| Noncoding strand | ||
| Donne-26 | 5′-GTAAGCGTCAGATAGTAACCAA-3′ | 638–617 |
| Donne-29 | 5′-GCGAAGCTTTTACTTCATAAAATATGTAAC-3′ | 855–835 |
| Donne-363 | 5′-CCTGAGTAAAACAGGATAC-3′ | 953–935 |
| Donne-382 | 5′-TCCTTCGGGTGATTGTGTA-3′ | 1300–1282 |
| Donne-416 | 5′-GTTGCGCTCATTACTTCT-3′ | 443–426 |
a
The underlined portion of each primer corresponds to the annealing site indicated within the bfp cluster nucleotide sequence (EMBL accession number Z68186).