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. 2022 Nov 30;20(12):757. doi: 10.3390/md20120757

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparing HPLC chromatogram of 10 and 100 pm caulerpin. Conditions: Inertsil C18 column (150 × 10 mm, 5 μm), mobile phase: MeOH: water (non-linear gradient; Turhan and Cavas, 2019) wavelength 235 nm, flow rate: 1 mL/min, column temperature: 25 °C injection volume: 50 μL, loop volume: 100 μL. Caulerpin was dissolved in methanol. (A) 10 ppm caulerpin filtered by using 0.45 μm membrane filter before injection; (B) 100 ppm caulerpin filtered by using 0.45 μm membrane filter before injection.