FIG. 6.
B. pertussis survives better in neutralized phagosomes, whereas elimination of B. bronchiseptica is enhanced. Bordetella-containing phagosomes were neutralized by ammonium chloride (A), bafilomycin A1 (B), or monensin (C). The time scale refers to the time after elimination of the extracellular bacteria by gentamicin treatment and extensive washing of the cells. At this time, the number of intracellular bacteria of each strain was set as 100%. Each experiment was carried out at least three times in duplicate, and the means of these data are presented. (D) For direct comparison of the effects of the various drugs, the fraction of surviving bacteria in the presence of the neutralizing drugs compared to surviving bacteria in the absence of these drugs (competition factor) is shown for each time.