TABLE 2.
B. pertussis and B. bronchiseptica colocalize with late endosomal/lysosomal macrophage proteins 4 h postinfection
| Antibody directed againstb: | % Colocalizationa
|
|
|---|---|---|
| B. pertussis | B. bronchiseptica | |
| LAMP-1 | 75 ± 5 | 74 ± 4 |
| Cathepsin D | 79 ± 7 | 83 ± 11 |
| v-ATPase | 69 ± 14 | 75 ± 10 |
| EEA1 | 3 ± 1 | 3 ± 2 |
| Transferrin receptor | 5 ± 2 | 4 ± 1 |
a
Numbers indicate the percentage of colocalization of vacuoles (stained with the respective antibody) and bacteria ± the standard deviation. The average of three experiments is shown, and 50 macrophages were counted each time.
b
LAMP-1, lysosome-associated membrane protein 1.