Figure 5.

Synergistic interactions between micellar curcumin and erufosine in ratio 4:1 lead to apoptotic cell dead in HuT-78 and MJ cells. Legend: (+) Co—cells incubated with hypertonic buffer; (+) Co/Inh—cells incubated with hypertonic buffer in the presence of caspase 3 inhibitor; (A)—caspase 3 activity of samples treated with ERF, MCRM or combinations; (B)—GSH levels in the same samples, presented in part A; (C)—accumulation of mono- and oligonucleotides in the cellular cytosol in the same samples presented in parts A and B; (D)—nuclear fragmentation after Hoechst staining of the cells in the same samples presented in parts A, B and C (depicted with white arrows). Significant differences between groups in (A,B) are marked with asterisks: *—p ≤ 0.05; **—p ≤ 0.01; ***—p ≤ 0.001, ****—p ≤ 0.0001.