Table 1.
Antibodies validated for bottlenose dolphin brains using immunohistochemistry (IHC) and Western blotting (WB) in this study.
| Antibody | Host | Reactivity | Clonality | RRID | Catalogue Number | Dilution IHC | Dilution WB |
|---|---|---|---|---|---|---|---|
| Aβ | rabbit | rat, human, mouse, bottlenose dolphin | monoclonal | AB_2532306 | 700254 | 1:500 | 1:500 1 |
| fibronectin | rabbit | human | polyclonal | AB_476976 | F3648 | 1:200 | 1:1000 |
| TDP-43 | rat | human, Rat | monoclonal | AB_2750118 | 829901 | 1:200 | 1:1000 |
| SMI-312 | mouse | human, mouse, rat | monoclonal | AB_2566782 | 837904 | 1:1000 | 1:1000 |
| Iba-1 | rabbit | human, mouse, rat, other | polyclonal | AB_839504 | 019-1974 | 1:500 | NA |
| GFAP | rabbit | cat, cow, dog, human, mouse, rat, sheep | polyclonal | AB_10013382 | Z0334 | 1:500 | 1:1000 |
1 Aβ-concentration in WB is derived from published work [5].