Figure 1.

ASFV-MNG19 Challenge Study Schematic. Six crossbred male pigs approximately 2-months-old were challenged with 360 HAD₅₀ of ASFV-MNG19. Samples were collected on days −1, 1, 3, 5, and 7 DPC. Samples collected included whole blood (EDTA), OP swabs, and oral fluids collected from chewed ropes at the pen level. Body temperature and clinical scores were assessed daily. One pig (#307) was necropsied on 5 DPC as a result of complications following sample collection. Another pig (#306) was found dead on 6 DPC and subsequently necropsied. The remaining 4 pigs were humanely euthanized and necropsied at 7 DPC due to severe clinical signs.