TABLE 1.
L. monocytogenes strains and plasmids used in this study
| Listeria plasmid or strain | Genotype or description | Source or reference |
|---|---|---|
| Plasmids | ||
| pAUL-A | Shuttle vector, thermosensitive, Em | 5 |
| pAUL-oppAΩaphA3′ | pAUL-A carrying oppAΩaphA3′ (2,331-bp fragment) | This study |
| pAT18 | Shuttle vector, Em | 41 |
| pAT18PdltA/oppA | pAT18 carrying oppA under control of the dltA promoter (2,035-bp fragment) | This study |
| Strains | ||
| LO28 | Virulent wild type, clinical isolate | 44 |
| LO28/pAT18 | LO28 harboring pATl8 (Em) | This study |
| LO28-oppA ΩaphA3′ | oppA mutant (Km) | This study |
| LO28-oppA ΩaphA3′/ pAT18 | oppA mutant harboring pAT18 (Em Km) | This study |
| LO28-oppA ΩaphA3′/ pAT18-PdltA/ oppA | oppA mutant harboring pAT18 with oppA under control of dltA promoter (Em Km) | This study |