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Osteogenic-specific gene expression by real-time quantitative polymerase chain reaction (RT–qPCR) analysis. RUNX2 (a), OPN (b), OCN (c) and BMP-2 (d) mRNA expression on Day 7, Day 14, and Day 21. Data represent mean ± standard deviation (n = 3). Significance was determined by one-way ANOVA (* p < 0.05, *** p < 0.001, **** p < 0.0001, ns: not significant vs. the 0 μg/mL group).
