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Expression of iNOS protein in alveolar macrophages was assessed by Western blot analysis with a monoclonal anti-iNOS antibody. U. urealyticum at a concentration of 4 × 108 CCU/ml stimulated iNOS protein expression, compared to unstimulated alveolar macrophages. iNOS protein expression was inhibited by budesonide (10−4 M) and dexamethasone (10−4 M).
