FIG. 4.
(A) Expression of iNOS protein in alveolar macrophages was assessed by immunostaining. U. urealyticum at a concentration of 4 × 108 CCU/ml stimulated iNOS protein expression, compared to unstimulated alveolar macrophages. iNOS protein expression was inhibited by budesonide (10−4 M) and dexamethasone (10−4 M). Magnification, ×500. (B) U. urealyticum antigen (4 × 108 CCU/ml) activated NF-κB expression after 30 min of incubation, compared to unstimulated alveolar macrophages. NF-κB expression was inhibited by budesonide (10−4 M) and dexamethasone (10−4 M). The intracellular location of p65 was detected by indirect immunofluorescence with an anti-p65 antibody. Magnification, ×500.