Figure 1.

Cell-surface characterization of 5-HT₂R-Dendra2 protein compared to 5-HT₂R-YFP in stable cell lines. (A) Representative images of stably-expressing 5-HT₂Rs-YFP, 5-HT₂Rs-Dendra2, and DRD2- Dendra2 constructs (green) in HEK293A cells, by confocal microscopy using a 60× objective lens (1.45 NA). Pan-cadherin (cell-surface marker) expression is shown in magenta, receptor in green and DAPI (nuclei) in blue. Scale bar = 10 µm. (B) Cell-surface expression levels for 5-HT₂R subtypes and DRD2-Dendra2 compared to 5-HT₂R subtypes-YFP were measured. Analysis was performed by quantifying Manders’ colocalization coefficient by measuring the percent colocalization of receptor expression with the pan-cadherin antibody (as surface marker) using ImageJ software. Percent cell-surface expression is averaged from at least 10 fields of view in three independent experiments. Error bars = SEM.