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. 2022 Nov 22;14(12):2597. doi: 10.3390/v14122597

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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JCPyV attachment is not enhanced in 5-HT₂R-expressing cells. Indicated stable cell lines were incubated with JCPyV-647 (filled histograms) or mock-treated (open histograms) on ice for 1 h and analyzed by flow cytometry. Histograms represent a comparison of JCPyV-647 attachment to HEK293A cells stably expressing 5-HT_2AR-Dendra2 (red), 5-HT_2BR-Dendra2 (orange), 5-HT_2CR-Dendra2 (green), DRD2-Dendra2 (blue), and Dendra2 (purple) proteins constructs. Histograms represent the mean fluorescence intensities for mock-treated (unfilled), or samples treated with JCPyV-647 (filled). Data are representative of 10,000 events for individual samples representative of 3 independent experiments. Data were analyzed using FlowJo software.