Figure 6.

5-HT₂R cluster properties for receptors subtypes in infected cells. Cluster properties including (A) mean cluster density, (B) circularity, and (C) area were calculated for localizations of 5-HT₂R-Dendra2 subtypes, DRD2-Dendra2 (Dopamine Dendra2), and Dendra2 in stable cells fixed at 0, 5, and 15 mpi and analyzed by widefield FPALM. Clusters were identified by binning molecules onto a grid and convolving with a circle (radius 50 nm) and thresholding for regions with density ≥3 times the cell average. The columns represent sample conditions, and rows represent the physical characteristics of clusters for each sample. In individual graphs, the y-axis indicates the value for each cluster characteristic, while the x-axis represents the 3 different time points. Cluster density is significantly increased at 5 mpi in cell types that support JCPyV infection. The Kruskal–Wallis test was used to compare the intensity values of each cluster property comparing all timepoints within each cell type. The symbol * indicates p < 0.05. Outliers are represented by black diamonds.