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. 2022 Dec 10;27(24):8770. doi: 10.3390/molecules27248770

Figure 2.

Figure 2

Immunomodulatory effects of PEGA on SCIC. Rats were immunized with SCH. SCIC were isolated on day 11 p.i. and cultivated in the absence (0) or presence of PEGA for 24 h, and cell viability was determined by MTT (A), cytokine levels were determined in cell culture supernatants by ELISA (B,C), DAF-FM was measured by fluorimetry (D), activation was assessed by OX40 (E) and CD25 staining (F) in CD4+ T cells by flow cytometry. Treg were identified as CD4+CD25+Foxp3+ cells (G). To determine Th17 (CD4+IL-17+) and Th1 (CD4+IFN-γ+) cells by flow cytometry, SCIC were stimulated with PMA and ionomycin in the presence of brefeldin A for 4 h before staining (H,I). Individual values of 5 (B,C), 4 (A,D–F) or 3 (H,I) samples are presented. In addition, the mean values +/− SD are given (A–D). One-way ANOVA followed by Tukey’s multiple comparison test (A–D), paired Student’s t-test (E–I), * p < 0.05, ** p < 0.01, *** p < 0.001, vs. 0.