Binding assay following PPE and PLE treatment. Binding experiments were carried out at 4 °C to allow cellular binding but not internalization of the virus and were performed under two conditions: (A) pretreatment of the virus before infection (virus pretreatment) and (B) pretreatment of cells with the extracts before infection with the virus (cell pretreatment). Vero cells and viral dilution were separately incubated for 1 h at 4 °C with or without PPE and PLE at 100 µg/mL and 50 µg/mL. Then, the infection was performed at a multiplicity of infection (MOI) of 1 with gentle shaking. After 1 h, the virus inoculum was removed, and the monolayers were differentially overlaid with culture medium containing 0.8% methylcellulose for 72 h for the viral plaque reduction assay. Viral plaques were visualized after 3 days using crystal violet staining and visualized with an inverted microscope (Leica DMIL, Nuβloch, Germany) for plaque morphology detection. Graphical representations of binding inhibition assay are shown on the right. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. HSV-1 + DMSO.