Table 1.
Tentative identification and quantification of polyphenols in pomegranate peel and leaf extracts (PPE and PLE, respectively) by LC-PDA-ESI-MS analysis.
| No. | Tentative Compound | [M − H]− | λmax | PPE | PPL |
|---|---|---|---|---|---|
| (m/z) | (nm) | mg/kg | |||
| 1 | Gallic acid | 169 | 215, 272 | 656.55 a | 2134.55 a |
| 2 | Catechin | 289 | 282 | 6578.22 b | - |
| 3 | Galloyl glucose | 331 | 278 | 8675.55 a | 568.99 a |
| 4 | Gallagyl-hexoside (Punicalin) | 781 | 266, 377 | 678.55 c | - |
| 5 | Granatin A | 783 | 272, 366 | 3088.77 c | - |
| 6 | Ellagitannin | 643 | 277 | 1088.44 d | - |
| 7 | Cyanidin 3,5-O-diglucoside | 611 | 515 | 58.78 e | - |
| 8 | Gallocatechin | 305 | 271 | 4558.77 b | - |
| 9 | Phellatin | 533 | 286, 332 | 4321.99 f | - |
| 10 | Granatin B | 951 | 276, 377 | 876.22 c | 143.66 c |
| 11 | Phlorizin | 435 | 280 | 44.88 f | 123.67 f |
| 12 | Coumaric acid-hexoside | 325 | 314 | 28.34 g | 121.88 g |
| 13 | Caffeoyl quinic acid | 353 | 212, 327 | - | 56.77 h |
| 14 | Dicaffeoylquinic acid | 515 | 221, 326 | - | 79.12 h |
| 15 | α-punicalagin | 1083 | 279 | 21,345.66 c | - |
| 16 | Hexahydroxydiphenichexoside | 481 | 267 | 123.44 c | - |
| 17 | Ellagic acid dihexoside | 625 | 288, 320 | - | 3245.66 d |
| 18 | Ellagic acid hexoside | 463 | 255, 362 | 18,765.33 d | 20,123.67 d |
| 19 | Feruloyl coniferin | 517 | 227 | - | 34.88 i |
| 20 | Delphinidin-3,5-O-diglucoside | 625 | 516 | 45.66 j | 21.33 j |
| 21 | Cyanidin pentoside | 417 | 280, 517 | - | 23.77 e |
| 22 | Cyanidin-3-glucoside | 449 | 274, 532 | 110.44 e | - |
| 23 | β-punicalagin | 1083 | 279 | 22,322.88 c | - |
| 24 | Pelargonidin | 271 | 230, 370 | - | 2.66 k |
| 25 | Delphinidin-3-O-glucoside | 465 | 527 | 7.88 j | - |
| 26 | Vanillic Acid hexoside | 329 | 268,300 | 654.33 l | - |
| 27 | Quercetin 3-O-rhamnoside | 447 | 265, 357 | 543.22 f | - |
| 28 | Ellagic acid deoxyhexoside | 447 | 257, 364 | 1234.77 d | 2467.88 d |
| 29 | Hexahydroxydiphenichexoside II (HHDP) | 481 | 268 | 897.66 c | - |
| 30 | bis-HHDP-hexoside (pedunculagin I) | 783 | 260, 382 | 3012.77 c | 534.12 c |
| 31 | Castalangin derivative | 965 | 277 | - | 377.88 d |
| 32 | Ellagic acid pentoside | 433 | 256, 362 | 11,234.66 d | - |
| 33 | cis-Dihydrokaempferol hexoside | 449 | 367 | 2134.76 m | - |
| 34 | trans-Dihydrokaempferol hexoside | 449 | 367 | 1657.22 m | - |
| 35 | Galloyl-HHDP-hexoside | 633 | 262, 335 | - | 1021.33 a |
| 36 | Galloyl-HHDP-gluconate | 649 | 267, 377 | - | 678.99 a |
| 37 | Digalloyl-HHDP-glucoside (Punigluconin) | 801 | 270, 377 | 1023.66 c | 145.66 c |
| 38 | Ellagic acid | 301 | 255, 367 | 13,245.66 d | 88.99 d |
| 39 | Dimethyl ellagic acid glucuronide | 505 | 272, 367 | - | - |
| 40 | Quercetin hexoside | 463 | 265, 357 | 3012.67 f | - |
| 41 | Kaempferol 3-O-glucoside | 447 | 265, 357 | 2134.22 m | - |
Polyphenols quantification was carried out by the built the following calibration curves: a gallic acid; b catechin; c α-punicalagin; d ellagic acid; e cyanidin 3-O-glucoside; f quercetin; g coumaric acid; h caffeic acid; i ferulic acid; j delphinidin-3-O-glucoside; k pelargonidin; l vanillic acid; m kaempferol-3-O-glucoside, respectively; -, not detected.