Table 2.
Differential metabolites after fatal intoxication by CPZ.
| Compound Name a | Metabolite Identification | P d | Trend e | VIP f | ||
|---|---|---|---|---|---|---|
| Identified Levels b | Accurate Mass c | RT c | ||||
| Chlorpromazine | 1 | 318.09555 | 10.888 | 2.82 × 10−8 | up | 1.783209 |
| L-Acetylcarnitine | 1 | 203.11574 | 9.1 | 5.77 × 10−7 | down | 1.687712 |
| Propionylcarnitine | 1 | 217.13124 | 9.878 | 5.77 × 10−7 | down | 1.45201 |
| Allantoin | 2 | 158.04388 | 0.921 | 7.66 × 10−7 | up | 1.703279 |
| Citric acid | 2 | 192.02698 | 1.526 | 0.000002 | up | 1.69585 |
| L-Carnitine | 2 | 161.10506 | 0.886 | 3.2 × 10−6 | down | 1.70532 |
| Docosahexaenoic acid | 2 | 328.24034 | 14.245 | 2.46 × 10−5 | up | 1.573368 |
| 3-Hydroxymethylglutaric acid | 2 | 162.05278 | 2.226 | 2.75 × 10−5 | up | 1.574867 |
| Oxoglutaric acid | 2 | 146.02148 | 1.526 | 4.38 × 10−5 | up | 1.561638 |
| Succinic acid | 1 | 118.02652 | 0.902 | 8.79 × 10−5 | up | 1.497697 |
| Gluconic acid | 2 | 196.05826 | 0.888 | 9.51 × 10−5 | up | 1.462974 |
| Kyotorphin | 2 | 337.1733 | 8.263 | 9.51 × 10−5 | down | 1.42069 |
| Threonic acid | 2 | 136.03716 | 0.911 | 9.51 × 10−5 | up | 1.473649 |
| Hexadecanedioic acid | 2 | 286.21451 | 12.576 | 0.000101 | up | 1.522403 |
| R-3-Hydroxydecanoic acid | 2 | 188.14122 | 11.839 | 0.000115 | down | 1.388931 |
| Dodecanedioic acid | 2 | 230.15171 | 10.764 | 0.000165 | down | 1.430021 |
| 3-Oxotetradecanoic acid | 2 | 242.18822 | 12.827 | 0.000329 | up | 1.445228 |
| Choline | 2 | 103.09959 | 0.868 | 0.000348 | up | 1.382057 |
| N-Acetyl-L-alanine | 2 | 131.05818 | 2.068 | 0.000617 | up | 1.339138 |
| Indolelactic acid | 2 | 205.07378 | 9.693 | 0.000747 | up | 1.352877 |
| L-Lysine | 2 | 146.10545 | 1.053 | 0.000747 | up | 1.399428 |
| 2-Octanamidoacetic acid | 2 | 201.13647 | 10.953 | 0.001291 | up | 1.296582 |
| Pipazethate | 2 | 399.1614 | 9.616 | 0.001915 | up | 1.217837 |
| Prostaglandin G2 2-glyceryl Ester | 2 | 442.25679 | 12.041 | 0.002218 | up | 1.263507 |
| Galactitol | 2 | 182.07882 | 0.89 | 0.004836 | up | 1.185343 |
| Indoxyl sulfate | 2 | 213.00958 | 6.979 | 0.005944 | up | 1.133043 |
| Hexanoylglycine | 2 | 173.10519 | 9.126 | 0.009866 | up | 1.086799 |
| L-Valine | 2 | 117.07885 | 0.926 | 0.023731 | down | 1.028871 |
a: The annotation for each compound is based on the HMDB database. b: The identification level of metabolites refers to the Metabolomics Standards Initiative reported by Sumner and coworkers. c: Accurate mass and RT were obtained from the mass spectrometer (Q Exactive) on full-scan mode. d: Calculated by Student’s t-test. Test level a = 0.05 and p < 0.05 means that the distribution of this metabolite between the fatal-intoxication group (at a high dose) and controls was significant. e: Comparison with controls. f: The VIP value is used to measure the ability of each metabolite to explain fatal intoxication, thereby assisting in the screening of marker metabolites (usually with VIP > 1.0 as the screening criterion).