Figure 4.

CEN modulates autophagy and senescence in SAOS400 cell lines. The cells were incubated for 120 h with cell culture medium, CEN, or Veh. Cell extracts were immunoblotted to detect autophagy and senescence markers, LC3I/II, Beclin-1 (panel (a,b)), and p16^INK4 (panel (c)), respectively. Densitometric analysis (numbers between panels indicate mean ± SD) was obtained normalizing the expression of LC3-II, Beclin-1, and p16^INK4 with the expression of α-tubulin, as described in the Materials and Methods Section. Symbols indicate significance: * p < 0.05 CEN with respect to Veh.