Table 2.
Primers designed for N gene amplification.
| Primer | Sense | Sequence | Size | Position * |
|---|---|---|---|---|
| N55 | F | 5′ ATGTAACACCTCTACAATGG 3′ | 842 bp | 55–74 |
| N879 ** | R | 5′ CAGGCTCGAACATTCTTC 3′ | 842 bp | 879–896 |
| N878 *** | R | 5′ CCCGGGCTCGAACATTCTTCT 3′ | 844 bp | 878–898 |
| N750 | F | 5′ GCACAGTTGTCACTGCTT 3′ | 793 bp | 750–767 |
| N1525B ** | R | 5′ GCACTTGGGCTGACAAAA 3′ | 793 bp | 1525–1542 |
| N1525D *** | R | 5′ TGCACTAGGATTGACAAAG 3′ | 793 bp | 1525–1542 |
* Position numbered according to RABV isolate Pasteur Virus (PV) sequence (GenBank Accession no. M13215). ** For amplification of bat-derived samples. *** For amplification of dog-derived samples. F—forward primer; R—reverse primer.