Table 1.
Primer sequences and annealing temperatures of the studied mouse templates.
Target | Forward primer | Reverse primer | Tann (°C) |
---|---|---|---|
MajorSR | 5′-GACGACTTGAAAAATGACGAAATC-3′ | 5′-CATATTCCAGGTCCTTCAGTGTGC-3′ | 57 |
MinorSR | 5′-CATGGAAAATGATAAAAACC-3′ | 5′-CATCTAATATGTTCTACAGTGTGG-3′ | 57 |
Line1 | 5′-TTTGGGACACAATGAAAGCA-3′ | 5′-CTGCCGTCTACTCCTCTTGG-3′ | 60 |
SineB1 | 5′-GTGGCGCACGCCTTTAATC-3′ | 5′-GACAGGGTTTCTCTGTGTAG-3′ | 60 |
SineB2 | 5′-GAGATGGCTCAGTGGTTAAG-3′ | 5′-CTGTCTTCAGACACTCCAG-3′ | 60 |
IAP | 5′-AGCAGGTGAAGCCACTG-3′ | 5′-CTTGCCACACTTAGAGC-3′ | 62 |
Gapdh | 5′-AGTGCCAGCCTCGTCCCGTA-3′ | 5′-AGGCGCCCAATACGGCCAAA-3′ | 57 |
18S | 5′-GTAGTCGCCGTGCCTACCAT-3′ | 5′-TTTTCGTCACTACCTCCCCG-3′ | 60 |
18S, 18S ribosomal DNA; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; IAP, intracisternal A particle; Line1, long interspersed nuclear element 1; Sine B1, short interspersed nuclear element B1; SR, satellite repeat; Tann, annealing temperature.