TABLE 1.

Reisolation of Campylobacter colonies from chicken ceca after colonization with the flaA mutant R1 and challenge with wild-type bacteria^a

Chicken no.	Kanamycin present in agar medium	No. of bacteria in cecum (CFU/g)
		Day 7	Day 15	Day 21	Day 29	Day 35	Day 42
1	Yes	9 × 107	<1 × 104	<1 × 103	1 × 108*	6 × 106*	1 × 106
	No	1 × 108	4 × 107	5 × 106	2 × 108*	4 × 108*	2 × 107
2	Yes	1 × 107	<1 × 104	<1 × 103	<1 × 103*	2 × 108*	<1 × 103
	No	2 × 107	1 × 106	4 × 108	5 × 108*	3 × 108*	2 × 106
3	Yes	2 × 107	<1 × 104	<1 × 103	<1 × 103	1 × 103	<1 × 103
	No	6 × 107	1 × 107	2 × 107	1 × 107	3 × 108	1 × 108
4	Yes	3 × 105	<1 × 104	<1 × 103	<1 × 103	1 × 104	2 × 108
	No	1 × 106	2 × 107	3 × 106	2 × 106	4 × 108	1 × 108
5	Yes	3 × 107	<1 × 104	<1 × 103	<1 × 103	<1 × 103*	<1 × 103
	No	2 × 107	1 × 107	3 × 107	2 × 108	1 × 108*	1 × 106
6	Yes	2 × 107	<1 × 104	<1 × 103	<1 × 103	1 × 107*	2 × 106
	No	4 × 107	<1 × 104	4 × 107	2 × 106	3 × 108*	2 × 106
7	Yes	2 × 107	1 × 105	<1 × 103	<1 × 103	1 × 105	<1 × 103
	No	1 × 107	1 × 107	3 × 109	4 × 103	1 × 109	2 × 108
8	Yes	1 × 107	<1 × 104	<1 × 103	<1 × 103	<1 × 103*	5 × 108
	No	2 × 107	2 × 107	5 × 107	1 × 109	2 × 108*	4 × 108
9	Yes	1 × 107	<1 × 104	6 × 108	<1 × 103	1 × 103	2 × 107
	No	1 × 107	2 × 108	2 × 108	2 × 108	1 × 108	1 × 108
10	Yes	4 × 107	<1 × 104	<1 × 103	<1 × 103	5 × 104*	<1 × 103
	No	4 × 106	1 × 107	1 × 107	4 × 107	1 × 108*	<1 × 103

^aBacteria giving values marked with an asterisk (two individual colonies and mixed cultures of more than 100 colonies) were analyzed at the DNA level by Southern blot analysis and PCR (see Table 2).