. Author manuscript; available in PMC: 2022 Dec 23.

Published in final edited form as: J Cell Physiol. 2019 Dec 25;235(6):5305–5317. doi: 10.1002/jcp.29416

Table 1.

Primer Sequences for qRT-PCR analysis in bovine disc cell culture.

Target	Forward Primer	Reverse primer	Primer Sequence
TNF-α	CACGTTGTAGCCGACATCAACTCT	GTTGTCTTCCAGCTTCACACCGTT	NM_173966.2
MMP-13	GCTTCCTGATGATGATGTCCAAGGGA	AGGGTCACATTTGTCTGGCGTT	NM_174389
NGF	GACCCACACCTTCGTCAAG	GTGTGTGCTCAGCAGGAA	NM_001099362
18s	GTAACCCGTTGAACCCCATT	CCATCCAATCGGTAGTAGCG	NR_003286

MMp-13: Matrix metallopeptidase 13; TNF-α: Tumor necrosis factor alpha; NGF: Nerve growth factor; CGRP: Calcitonin Gene-related Peptide; 18s: 18S ribosome.