| Steps | Temperature | Duration | Cycles |
|---|---|---|---|
| UDG activation | 50 °C | 2 min | |
| Dual-Lock™ Taq DNA polymerase | 95 °C | 2 min | |
| Denaturation | 95 °C | 15 s | 40 |
| Annealing/ extend | 60 °C | 1 min |
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