| Step | Ramp rate | Temperature | Time |
|---|---|---|---|
| Denaturation | 1.6 °C/s | 95 °C | 15 s |
| Annealing | 1.6 °C/ s | 60 °C | 1 min |
| Dissociation | 0.15 °C/ s | 95 °C | 15 s |
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