FIG. 3.

Antigen recognition by CD8⁺ T cells from BCG-vaccinated donors and TB patients. PBMC from 10 healthy donors (A) and 10 TB patients (B) were incubated in the presence of either M. bovis BCG (open bars) or M. tuberculosis H37Ra (solid bars) for 7 days. CD8⁺ T cells were purified by MACS. Autologus monocyte-derived macrophages were infected overnight with either M. bovis BCG, M. tuberculosis H37Ra, or rVV-expressing mycobacterial antigens. Effector cells were incubated with autologous, antigen-pulsed macrophages for 6 h at a 30:1 E/T ratio. CTL activity was measured as reflected by specific ⁵¹Cr release. The results are expressed as the mean percent specific lysis ± the standard deviation.