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. 2022 Dec 16;12(12):1280. doi: 10.3390/metabo12121280

Table 1.

Carcinogenic roles of lipid in glioma.

Research Diagnosis Experimental Model Sample Type Analytical Platform Lipid Species Physiological Effect on Glioma
Fatty Acyls (FA)
[20] GBM Human Serum GC-TOFMS ↑ Butyric acid (C4) Provide substrate for glutamate metabolism
[21] GBM Cell line Tissue GCMS ↑ Octanoic acid (C8) Activated ketone body metabolism for glioma cell survival
[21] GBM Cell line Tissue GCMS ↑ Decanoic acid (C10) Stimulate fatty acyl production
[22] GBM Human Tissue GC-TOFMS ↓ Lauric acid (C12) Tumour malignancy
[23] GBM Cell line Tissue HPLC,
Spectrophotometric
↑ Palmitic acid (C16) Enhanced glioma cell proliferation
[20] GBM Human Serum GC-TOFMS ↑ Stearic acid (C18:0) Provide substrate for glutamate metabolism
[24,25,26] Oligodendroglioma
Astrocytoma
GBM, glioma
Cell line Tissue Raman
spectroscopy MS
↑ Oleic acid (C18:1) Cellular apoptosis, Enhance proliferation of GBM cells
[20] GBM Human Serum GC-TOFMS ↑ Linolenic acid (C18:3) Provide substrate for glutamate metabolism
[20] GBM Human Serum GC-TOFMS ↑ AA (C20:4) Provide substrate for glutamate metabolism
[27] GBM Human Serum MS ↓ VLCDCA Anti-inflammatory and has chemopreventative properties
[28] Glioma (Grade II, III), GBM Human Serum LCMS/MS ↑ Stearoylcarnitine (C18), margaroylcarnitine,
Eicosenoylcarnitine (C20:1)
Supply substrate for the activation of fatty acyl metabolism
[29] GBM Cell line Tissue HPLC-MS/MS ↑ PGD2 Support glioma growth and invasion
[30]
[31]
Glioma
GBM
Cell line
Human & animal
Tissue Biochemical assay ↑ PGE2 Protect glioma cells against radiation treatment. Escalate self-renewal capacity and resistance to radiation-induced DNA damage
Glycerolipid (GL)
[32] LGG, HGG Human Tissue LCMS ↑ 2-AG Inhibit cell apoptosis, support cell proliferation and survival
[33] Grade III Human CSF MS/MS ↑ DG Malignant transformation
[21,34] GBM Cell line Tissue GCMS ↓ DG
(DG34:0, DG34:1, DG36:1, DG38:4, DG38:6, DG40:6)
Influence carcinogenesis signaling and inflammatory reaction in GBM
[35,36] GBM Cell line Tissue Microscopy ↓ TG, LD Mitochondria hydrolyzed lipid droplets and utilized triglycerides for energy production
[37] Medulloblastoma Human Tissue Raman Imaging ↑ TG Tumour development
Glycerophospholipid (GP)
[38,39] GBM Cell line Tissue Biochemical assay, UPLC-MS/MS ↑ PA Lipid signaling of autophagy and cell survival in glioma
[37,40] GBM, glioma Animal, human Tissue MALDI-IMS ESI-MS/MS, Raman imaging ↓ PA
(PA36:2, PA42:5, PA42.7)
↓ DHA
Influence carcinogenesis signaling and inflammatory reaction in GBM
[41] IDHwt glioma Cell culture Tissue NMR ↑ PC Increase cell turnover and tumour growth
[34,42,43,44,45,46,47]
[28,48,49,50,51,52]
GBM
Astrocytoma, GBM
Cell line
Human
Tissue
Tissue, Serum
NMR
LCMS
↑ PC
↑ PC
PC14:2
Malignant progression and aggressiveness in GBM
[26,34,48]
[33]
Glioma
Grade III
Human Tissue
CSF
Swab TS-MS, LCMS
MS/MS
↑ PI, PG Enhanced tumour infiltration
[34,53] GBM Cell line Tissue MSI, LCMS ↑ PE Enhanced tumour growth
[41] IDHwt glioma Cell line Tissue NMR ↑ PE Increase cell turnover and tumour growth
[54] GBM Animal Tissue MRI ↓ LPA Support cell proliferation through the disassembling of primary cilia
[55,56] Glioma Grade II, III
GBM
GBM
Human
Cell line
Tissue GCMS. LCMS ↓ LPC, LPE Provide substrates to
mitochondria to generate energy
Sphingolipid (SP)
[57,58,59,60,61,62,63,64,65] GBM Cell line Tissue Biochemical assay ↑ S1P Resistant to chemo-therapeutic treatment and sustain the growth of glioma cells. Induced cell angiogenesis
[66,67]
[33]
GBM
Grade III
Cell line
Human
Tissue
CSF
Biochemical assay
MS/MS
↑ S1P, ↑ Cer, ↑ SM GBM cell proliferation
[68] Oligodendroglioma Cell line Tissue LCMS ↑ NDMS,
↑ Sphingosine-C18,
↑ Sphingosine C17, ↑ Sphinganine C17
Signaling roles for proliferation and survival
[28,69] Glioma Grade II-III and GBM Human Serum
Tissue
LCMS/MS,
MALDI-TOF-MS
↑ SM (d16:1/23:0, d17:1/18:0, d18:1/17:0, d18:0/15:0, d18:1/16:0, d18:0/17:0, d19:1/16:0) Involved in the regulation of sphingolipid metabolism and malignancy of glioma, glioma cell senescence and apoptosis
[33] Grade III Human CSF HPLC/MS ↑ N-Lignoceroylsphingosine Involved in lipid signaling and apoptosis
[70] GBM Human Tissue Biochemical assay ↑ OAcGD2 Increase tumour density and involvement in immunoresistance
[71] Diffuse midline glioma Cell line Tissue Biochemical assay ↑ GD2 Enhanced metastasis
[72,73,74] GBM, anaplastic oligodendroglioma Animal Tissue Biochemical assay ↑ GD3 Involved in cell transformation and malignancy
[75] GBM Cell line Tissue Biochemical assay ↑ GM2 Enhanced cell migration
[76] Glioma, Medulloblastoma Animal Tissue MALDI-IMS ↑ GM3 Induced malignancy, invasiveness and progression of tumour
Sterol Lipid (ST)
[77] GBM Cell line Tissue Biochemical assay ↑ CE Tumour progression and malignant
[33] Grade III Human CSF HPLC/MS ↑ 1-Oleyl-cholesterol Enhance tumour growth
[33] Grade III Human CSF HPLC/MS ↑ Tetrahydrocorticosterone Enhanced metastasis
[78,79] GBM Cell line Tissue GCMS
Biochemical assay
↑ 24S-OHC Induced tumour growth by regulating proinflammatory immune cells.
nduced pathogenesis of tumour cells
[80] GBM Human Serum Biochemical assay LDL Support proliferation and growth of glioma

↑ denotes increased levels and ↓ denotes decreased levels. AA arachidonic acid; CE cholesterol ester; Cer ceramide; DG diacylglycerol; DHA docosahex-aenoic acid; GBM glioblastoma; GC-TOFMS gas chromatography-time of flight- mass spectrom-etry; GCMS gas chromatography mass spectrometry; GD2 disialoganglioside; GD3 ganglioside GD3; GM2 ganglioside M2; GM3 ganglioside GM3; HGG high grade glioma; HPLC high per-formance liquid chromatography; HPLC/MS high performance liquid chromatography—mass spectrometry; HPLC-MS/MS high performance liquid chromatography with tandem mass spec-trometry; IDHwt isocitrate dehydrogenase (IDH)-wildtype; LCMS liquid chromatography mass spectrometry; LCMS/MS liquid chromatography with tandem mass spectrometry; LD lipid droplet, LDL low density lipoprotein; LGG low grade glioma; LPA lysophosphatidic acid; LPC lysophosphatidylcholine; LPE lysophosphatidylethanolamine; MALDI-IMS matrix-assisted laser desorption ionization—imaging mass spectrometry; MALDI-TOF-MS matrix-assisted laser de-sorption ionization time-of-flight mass spectrometry; MS mass spectrometry; MS/MS tandem mass spectrometry; MRI magnetic resonance imaging; NMR nuclear magnetic resonance; OAcGD2 O-Acetyl-ganglioside 2; PA phosphatidic acid; PC phosphatidylcholine; PE phosphati-dylethanolamine; PG phosphatidylglycerol; PGD2 prostaglandin D2; PGE2 prostaglandin E2; PI phosphatidylinositol; S1P sphingosine-1-phosphate; SM sphingomyelin; Swab TS-MS swab by using touch spray-mass spectrometry; TG triacylglycerol; VLCDCA Very long chain dicarbox-ylic acids; 2-AG 2-arachidonoylglycerol; 24S-OHC 24(S)-hydroxycholesterol.