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. 2022 Dec 23;864:161152. doi: 10.1016/j.scitotenv.2022.161152

Table 2.

Early warning methods.

Type of analysis Early warning parameter Early warning level / cutoff level Abbreviation Data type

Real-time analysis
Data sequence numerical increase or decrease Keep increasing for 3 consecutive data points OBMN1N2 N1 N2 gene concentrations
Positive percentage change > 40 % PPCN1N2 N1 N2 gene concentrations
Positive percentage change of slope > 200 % PPCS200 N1 N2 gene concentrations



Post-factum analysis
Mean + 2 standard deviation Intersection values higher than mean + 2SD threshold MSD (B1) N1 N2 gene concentrations
and
w/c ratio
Variance / mean Intersection values higher than variance/mean threshold VAM (B2)
Skewness Intersection values higher than skewness threshold SKE (B3)
Kurtosis Intersection values higher than kurtosis threshold KUR (B4)
90 percentile Intersection values higher than 90 percentile threshold PER90 (B5)

Note: (1) B1, B2, B3, B4, B5 are short representation of each statistical method for visualization purposes. (2) * marked methods were demonstrated in figures in the main text.