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. 2022 Dec 17;23(24):16123. doi: 10.3390/ijms232416123

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic analysis, spatial and temporal expression profile and subcellular localization of ZmBZ1. (A) Protein structure of ZmBZ1; (B) Phylogenetic analysis of ZmBZ1 and its homologs in different species. Red triangle protruding ZmBZ1. Bar = 0.2 substitutions per amino acid position; (C,D) Spatial and temporal expression profile of ZmBZ1 analyzed via qPCR in various tissues (C) and during seed development (D). GAPDH was used as an internal control. Values are shown as means ± SE, n = 3; (E,F) Subcellular localization of ZmBZ1 protein in maize leaf protoplasts (E) and tobacco leaf epidermal cells (F). GFP, green fluorescence protein; YFP, yellow fluorescence protein; Athook, a nuclear marker. Bar = 5 μm and 10 μm.