Table 1.
Arrangements determined by long‐range PCR
| Variant identifier | Informative products | Noninformative products | Genome arrangement | Colony morphology 3 |
|---|---|---|---|---|
| 7 | 3/8, 4/7, 5/6, 9/10, 11/12, 13/14, 15/16 | 3/5, 8/11 1 | 17ʹ35642ʹ (GS2.66) | Large colonies(∼2–3 mm) |
| 8 | 3/8, 4/7, 5/6, 9/12, 10/11, 13/14, 15/16 | 6/13 1 , 8/11 1 | 1ʹ24ʹ3567 (GS19.9) | Large colonies (∼2–3 mm) |
| U | 3/8, 5/6, 11/14, 12/13, 15/16 | 8/11 1 | 1ʹ24ʹ6ʹ5ʹ37 (GS2.57) | Medium size colonies (∼1–2 mm) |
| T | 3/8, 4/11, 5/6, 7/10, 7/12, 9/10, 12/13, 13/14, 15/16 | 135642ʹ7 (GS21.3) and 1ʹ6ʹ5ʹ35642ʹ7 2 | Small, pin‐prick colonies (≤1 mm) |
Primer combinations resulting in PCR products that gave an informative 6 kb band were used to determine genome structures. Primer combination products were deemed noninformative either due to spurious bands of incorrect size or representing circularized fragments.
1
8/11 = circularized fragment 6; 6/13 = circularized fragment 5;
2
No GS assigned because the structure includes duplicated fragments.
3
Parent strain WT was large colonies (2–3 mm); morphology images in Figure S1.