Figure 2.
Effect of BIO-PLYTM treatment on PRRSV infection of porcine alveolar macrophages (PAM) and pulmonary intravascular macrophages (PIM). Briefly, PAM and PIM were infected with PRRSV with and without BIO-PLYTM treatment, and viral copies produced after 24 h of infection were measured via RT-qPCR. All experiments were performed using technical duplicates in 3 biological replicates. Obtained values were normalized to the average values for each of the three PRRSV strains without BIO-PLY treatment (set as 1) to reduce variation between biological samples and visualized as proportions based on the average values of infection. Results were analyzed via two-way ANOVA and displayed showing means and standard error of mean (SEM). p-values were expressed using asterisks following APA and Prism standards. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.