Figure 6.
Effect of BIO-PLYTM treatment on the ROS production of porcine alveolar macrophages (PAM) infected with PRRSV-2. Briefly, cells were infected with (A) PRRSV NC-134, (B) PRRSV NC-174, or (C) PRRSV VR-2332, or left in culture alone (mock). ROS production was measured at different time points (left) and visualized at 48 h (right). All experiments were performed using technical duplicates in 4 biological replicates. Obtained values were normalized to the average of the mocks. Results were analyzed via ANOVA and displayed showing means and standard error of mean (SEM). p-values were expressed using asterisks following APA and Prism standards. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001.