Figure 4.

SA is involved in grafting-induced accumulation of ABA and H₂O₂ in cucumber leaves. (a,b) Changes of NCED mRNA abundance and ABA content in Cs/Cs, respectively, at 25 °C. (c,d) Changes of RBOH1 mRNA abundance and H₂O₂ content in Cs/Cs, respectively, at 25 °C. Plants with three leaves were pretreated with 1.0 mM SA or distilled water (control) for 12 h. (e,f) ABA and H₂O₂ contents in Cs/Cs and Cs/Cm leaves, respectively, before and after exposure 5 °C for 24 h. Plants were pretreated with 1.0 mM SA, 30 μM AIP, 0.1 mM AOPP (two biosynthetic inhibitors of SA), or deionized water (control), respectively, for 12 h and then exposed to chilling stress. Data are presented as the means ± SD (n = 3). Different letters indicate a statistical significance between samples at p < 0.05.