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. 2022 Dec 16;23(24):16057. doi: 10.3390/ijms232416057

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of ABA on the H₂O₂ biosynthesis and the role of H₂O₂ in ABA−induced chilling tolerance in grafted cucumber. (a,b) RBOH1 mRNA abundance and H₂O₂ accumulation, respectively, in Cs/Cs leaves. The Cs/Cs plants were pretreated with 50 μM ABA or distilled water (control), and then we measured the changes of RBOH1 mRNA abundance and H₂O₂ content within 12 h. (c) H₂O₂ accumulation in Cs/Cs or Cs/Cm after chilling treatment for 0 h and 12 h. Plants were foliar sprayed with 50 μM ABA, 3 mM Na₂WO₄, 50 μM Flu, or distilled water (control) for 12 h and then exposed to chilling stress. (d) Electrolyte leakage of Cs/Cs or Cs/Cm plants after chilling treatment for 0 h and 48 h. (e) Chilling injury index of Cs/Cs or Cs/Cm plants after chilling treatment for 0 h and 72 h. (f) F_v/F_m in Cs/Cs or Cs/Cm plants after chilling treatment for 0 h and 48 h. Plants were pretreated with 50 μM ABA, 5 mM DMTU + 50 μM ABA, 0.1 mM DPI +50 μM ABA, or distilled water (control) for 12 h and then exposed to chilling stress. Data are presented as the means ± SD (n = 3). Different letters indicate a statistical significance between samples at p < 0.05.