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. 2022 Dec 6;12(12):1224. doi: 10.3390/metabo12121224

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phenylacetylglutamine may predict parasitemia in Africans before PfSPZ DVI. Confirmation of the identity of phenylacetylglutamine (PAG) with the analysis of a sample composed of pooled urine samples collected from TBS negative Africans at Day-1 before PfSPZ DVI (Blank urine), the same urine sample spiked with PAG (Spiked urine) and a neat standard of PAG (PAG standard). (A) Extracted ion chromatogram, (B) MS spectrum and (C) MS/MS fragmentation spectrum, of PAG in blank urine, spiked urine and PAG standard. The retention times, as well as MS and MS/MS spectra of PAG overlap in the different samples injected, confirming the identity of PAG (confidence level 1 in the metabolite identification). Experimental conditions: see main text.