Figure 3.

Analysis data of next-generation sequencing (NGS) and third-generation sequencing (TGS)-derived DEGs in 93-11D and 93-11T with or without Na₂CO₃ treatment. (A) Hierarchical clustering of 24 samples according to the correlation coefficient between each sample. (B) Heat map hierarchical clustering of the overall gene expression. (C) Statistics of up- (in red) and down-regulated (in blue) DEGs in leaf, root and leaf vs. root for each pairwise comparison. Venn diagrams of up- and down-regulated DEGs in leaves (D), roots (E), leaf/root of diploid (F), and leaf/root of tetraploid (G). DC and TC represent diploid and tetraploid without stress, respectively; DS and TS represent diploid and tetraploid under Na₂CO₃ stress, respectively.