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. 2022 Dec 1;14(12):2694. doi: 10.3390/pharmaceutics14122694

Figure 5.

Figure 5

In vitro cytotoxicity in HEK 293T cells of citrate-functionalized Ca-Mn ferrite NPs. Cell viability was assessed by MTT assay in HEK 293T cells incubated with four different concentrations of NPs (100, 150, 200, 250 µg/mL) for 24 h (a) and 48 h (b). Untreated cells at 24 h and 48 h were used as a negative control (100% viable cells). Data are expressed as the mean of three independent experiments (n = 3). Two-way ANOVA assessed by Tukey’s multiple comparison test indicates no statistically significant differences between the groups tested, denoted as ns p > 0.05. Abbreviations: x = 0 + cit, x = 0.2 + cit and x = 1 + cit are citrate-functionalized MnFe2O4, Ca0.2Mn0.8Fe2O4 and CaFe2O4 nanoparticles, respectively.