Figure 1.

Ribosome display cycle. DNA encoding in library was first transcribed, and resulting mRNA was translated by PUREfrex^®RD cell-free translation system optimized for ribosome display, to give rise to mRNA–ribosome–peptide complexes through SecM arrest sequence, which acts within the ribosomal exit tunnel to stop translation. mRNA–ribosome–peptide complexes were used directly for selection against an immobilized target on beads. After selection, retained complexes were disrupted by the addition of EDTA. mRNA was isolated, reverse transcribed, and amplified for the next round.