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. 2022 Dec 2;15(12):1506. doi: 10.3390/ph15121506

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of interaction between CTLA-4 and B7-1 by C4-302. Inhibitory activity of C4-302 was examined. C4-351, which was found to bind to Fc region of CTLA-4, was used as negative control. After mixing mRNA–ribosome–peptide complex of each clone with CTLA-4, B7-1 immobilized on streptavidin magnetic beads via biotin was added to the mixture. (A) Because C4-351 bound to the Fc region of CTLA-4, mRNA was collected at nearly 10¹⁰. (B) In C4-302, mRNA collection was reduced considerably to just under 10⁷. This finding suggests that the C4-302 mRNA–ribosome–peptide complex inhibited the interaction of CTLA-4 with B7-1.