Figure 3.

Exogenous expression levels of SOD1-myc and nanobody proteins are positively correlated in cellulo. Linear-regression analyses of SOD1 and nanobody signal intensity. For all conditions there is a moderate positive correlation, with SOD1 and Nb54-PEST co-transfections demonstrating the strongest correlations. (A) HEK293T cells co-transfected with SOD1 WT and Nb54, Nb54-PEST, Nb61, or Nb61-PEST. (B) HEK293T cells co-transfected with SOD1 A4V and Nb54, Nb54-PEST, Nb61, or Nb61-PEST. (C) HEK293T cells co-transfected with SOD1 G93A and Nb54, Nb54-PEST, Nb61, or Nb61-PEST. For (A–C): Data are pooled from three biological replicates with the exception of SOD1 WT co-transfected with Nb61 and Nb61-PEST, which are pooled from six biological replicates. Each point represents one cell; each symbol denotes a specific biological replicate. The r² values reflect the fit of experimental data to the depicted regression line. Increasing r² values indicate that the SOD1 and Nb signal intensities are positively correlated.