Table 7.
Acute toxicity assay of X. spinosum ethanol extract, aqueous and aq. Methanol fractions in mice.
| Groups (n = 6) | Dose (mg/kg) | Dose Difference (a) |
Ethanol Extract | Aqueous & aq. Methanol Fractions | ||||
|---|---|---|---|---|---|---|---|---|
| No. of Mortality | Mean Mortality (b) | Probit (a × b) | No. of Mortality | Mean Mortality (b) | Probit (a × b) |
|||
| 1 | 500 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 2 | 4000 | 3500 | 2 | 1 | 3500 | 1 | 0.5 | 1750 |
| 3 | 7500 | 3500 | 3 | 2.5 | 8750 | 3 | 2 | 7000 |
Mice (n = 6) were injected intraperitoneally and observed for 24 h. n = number of mice in each group. Dose difference (a) = higher dose − lower dose. Mean mortality (b) = (mortality in the second concentration + mortality in the first concentration)/2.