Table 4.
Effect of different concentrations of cotton seed cake extract on the mortality of J2s of M. incognita over 12, 24, 36, and 48 h of incubation in vitro.
| Treatment | Concentration (mg/L) |
Number of Dead J2s (Mean ± SE) at Different Time Intervals (Hours) | |||
|---|---|---|---|---|---|
| 12 | 24 | 36 | 48 | ||
| Cotton seed cake | 250 | 8 c ± 1.53 (8.89%) |
13 c ± 1.53 (14.44%) |
19 d ± 1.53 (21.11%) |
30 c ± 2.08 (33.33%) |
| 500 | 12 c ± 2.08 (13.33%) |
18 c ± 2.52 (20.00%) |
30 c ± 2.31 (33.33%) |
47 b ± 2.08 (52.22%) |
|
| 750 | 17 b ± 1.53 (18.89%) |
25 b ± 2.31 (27.78%) |
39 b ± 2.31 (43.33%) |
54 b ± 1.53 (60.00%) |
|
| 1000 | 25 a ± 2.08 (27.78%) |
37 a ± 1.73 (41.11%) |
51 a ± 3.21 (56.67%) |
67 a ± 2.52 (74.44%) |
|
| DW | 0 d ± 0 (0.00%) |
0 d ± 0 (0.00%) |
0 e ± 0 (0.00%) |
0 d ± 0 (0.00%) |
|
| Df | 4 | 4 | 4 | 4 | |
| Sum of Squares | 1059.60 | 2271.60 | 4556.40 | 8019.60 | |
| Mean Squares | 264.90 | 567.90 | 1139.10 | 2004.90 | |
| F-Calculated | 36.79 | 74.72 | 81.36 | 123.75 | |
| Significance | 0.00001 | 0.0000 | 0.0003 | 0.0002 | |
Each value is an average of five replicates. DW = Double Water (control). SE—Standard Error. J2s—second-stage juveniles. Values given in parentheses represent percent J2 mortality over control. Values given without parentheses represent number of the dead J2s of M. incognita in different concentrations. Values followed by the same letter within a column, are not significantly different according to Duncan’s multiple-range test (p ≤ 0.05).